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American Society of Microbiology ABMM Sample Questions:

1. A Gram stain of anaerobic growth from a peritoneal fluid abscess reveals pleomorphic, pale-staining Gram- negative rods. The isolate grows on Bacteroides Bile Esculin (BBE) agar, hydrolyzing esculin (producing black colonies), and is resistant to kanamycin (1000 µg), vancomycin (5 µg), and colistin (10 µg) disks. Which organism group is most likely?

A) Bacteroides fragilis group
B) Prevotella melaninogenica group
C) Fusobacterium nucleatum
D) Clostridium perfringens

2. For epidemiological tracking and outbreak investigations of Clostridioides difficile infection (CDI), which molecular typing method based on PCR amplification targeting repetitive elements within the pathogenicity locus (PaLoc) has historically been widely used and designated by specific nomenclature (e.g., NAP1/BI
/027)?

A) Multilocus sequence typing (MLST)
B) PCR Ribotyping
C) Pulsed-field gel electrophoresis (PFGE)
D) Whole-genome sequencing (WGS) SNP analysis

3. During the investigation of a foodborne illness outbreak linked to raw sprouts, several bacterial isolates are obtained from patient stool samples and the implicated food source. Which of the following molecular methods would be MOST useful for establishing a definitive link between the clinical isolates and the food isolate?

A) Pulsed-field gel electrophoresis (PFGE)
B) Serotyping based on O and H antigens
C) Gram staining and biochemical profiling
D) Antimicrobial susceptibility testing

4. A patient develops a severe gastrointestinal illness after consuming undercooked poultry. Stool culture yields a Gram-negative, microaerophilic, curved bacillus that is oxidase-positive. The MOST likely organism is:

A) Salmonella enterica
B) Shigella dysenteriae
C) Campylobacter jejuni
D) Yersinia enterocolitica

5. A research laboratory is investigating the mechanisms of antiviral drug resistance. They are studying a herpes simplex virus strain that has developed resistance to acyclovir. The MOST common mechanism of acyclovir resistance in HSV involves mutations in the gene encoding:

A) Viral protease
B) Viral thymidine kinase
C) Viral DNA polymerase
D) Viral glycoprotein D

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